British schoolgirls (from left) Kadiza Sultana, Amira Abase and Shamima Begum are believed to be in Syria.
Mark Keary of Bethnal Green Academy, east London, said that the school was "shocked and saddened" by the disappearance of Kadiza Sultana, 16, and 15-year-olds Amira Abase and Shamima Begum.
On February 15 Ms Begum used Twitter to get in touch with 20-year-old Aqsa Mahmood, a woman from Glasgow who is understood to have joined Isis and married one of its fighters. Mr Keary said that access to social media at the school is "strictly regulated".
"With such measures in place, police have advised us that there is no evidence that radicalisation of the missing students took place at the academy," he said.
It emerged on Friday that the three missing girls were close to a 15-year-old from their school who travelled to Syria last December.
Mr Keary said police had spoken to the girls after the previous student disappeared and indicated at the time that there was no evidence that they were at risk of being radicalised or absconding.
There was growing concern about the role played by airport and border staff after it emerged Ms Begum used the passport of her 17-year-old sister, Aklima, to leave the UK.
David Cameron has called for airlines and internet firms to be more proactive in curtailing radicalisation.

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